Journal: Nutrition Research and Practice

Article Title: Modulatory effects of Hizikia fusiformis on the dopaminergic system in a neonatal habenular lesion-induced attention-deficit hyperactivity disorder-like rodent model

doi: 10.4162/nrp.2026.20.1.31

Figure Lengend Snippet: (A) The bar graph indicates the percentage of VEH in HF-induced MAP2 expression. (B, C) These bar graphs are similar to graph (A); however, they show the expression of NF (B) and SYP (C). Protein expression was analyzed using analysis of variance and Bonferroni post-hoc test. Statistical significance was set at P < 0.05. HF, Hizikia fusiformis ; MAP2, microtubule-associated protein 2; NF, neurofilament; SYP, synaptophysin; VEH, vehicle.

Article Snippet: The following were the primary antibodies used: mouse monoclonal anti-synaptophysin (SYP; catalog #: S5768; Sigma-Aldrich), rabbit polyclonal anti-tyrosine hydroxylase (TH; catalog #: E-AB-33093; Elabscience, Houston, TX, USA), mouse monoclonal neurofilament (NF; catalog #: sc-20012; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-DAT (catalog #: PA1-4656; Invitrogen, Waltham, MA, USA), mouse monoclonal anti-DRD2 (catalog #: sc-5303; Santa Cruz Biotechnology), rabbit polyclonal anti-DRD1 (catalog #: E-AB-31152; Elabscience), mouse monoclonal anti-DRD3 (catalog #: sc-136170; Santa Cruz Biotechnology), rabbit polyclonal anti-DRD5 (catalog #: MBS2516950; MyBioSource, San Diego, CA, USA), mouse monoclonal anti-microtubule-associated protein 2 (MAP2; catalog #: sc-32791; Santa Cruz Biotechnology), and rabbit polyclonal anti-DRD4 (catalog #: E-AB-31153; Elabscience).

Techniques: Expressing

Journal: Journal of Molecular and Cellular Cardiology Plus

Article Title: Independent and synergistic roles of MEK-ERK1/2 and PKC pathways in regulating functional changes in vascular tissue following flow cessation

doi: 10.1016/j.jmccpl.2025.100300

Figure Lengend Snippet: Signaling pathways involved in ET B receptor upregulation and VSMC contraction. Extracellular stimuli such as flow cessation activate the MAPK/ERK pathway, which is disrupted by MEK inhibition (Trametinib) or ERK inhibition (Ulixertinib). In parallel, intracellular Ca 2+ increase leads to delayed PKC activation, promoting NF-κB signaling via the IKK complex. PKC and NF-κB inhibition (RO-317549 and BMS345541, respectively) remain effective when applied up to 6 h post-stimulus. Combined inhibition further suppresses ET B upregulation on VSMCs and reduces receptor-mediated contraction, indicating that both pathways contribute independently and are required for full functional response . Abbreviations: ET B : endothelin type B receptor; VSMC: vascular smooth muscle cell; NF-κB: nuclear factor. Created in BioRender. Kazantzi, S. (2025) https://BioRender.com/u89c445

Article Snippet: The membranes were then transferred to a rocking table in room temperature with Blocking Buffer (EveryBlot Blocking Buffer, Cat. #: 12010020, Bio-Rad) for 10 min and then, the membranes were incubated with primary antibody dilutions overnight in 4 °C (1:1000 Mouse Anti-p44 / 42 MAP Kinase Monoclonal Antibody, Unconjugated, Clone L34F12 [Cell Signaling Technology Cat# 4696, lot: 29, RRID: AB_390780 ], 1:1000 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb [Cell Signaling Technology Cat# 4370, Lot: 28, RRID: AB_2315112 ), 1:1000 NF-κB p65 (L8F6) Mouse mAb [Cell Signaling Technology Cat# 6956, Lot:10, RRID: AB_10828935 ], 1:1000 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb [Cell Signaling Technology Cat# 3033,Lot: 19, RRID: AB_331284 ], GAPDH (D16H11) XP Rabbit mAb, [Cell Signaling Technology Cat# 5174, Lot: 8, RRID: AB_10622025 ], 1:1000 Actin, Smooth Muscle (1A4) Mouse Monoclonal Antibody [Cell Marque Cat# 202 M-94, Lot:0000243213, RRID: AB_1157937 ], 1:1000 alpha smooth muscle Actin antibody (EPR5368) [Abcam Cat# ab124964, Lot: GR303485 –26, RRID: AB_11129103 ]).

Techniques: Protein-Protein interactions, Inhibition, Activation Assay, Functional Assay

Journal: Cells

Article Title: Small Extracellular Vesicles Promote Axon Outgrowth by Engaging the Wnt-Planar Cell Polarity Pathway

doi: 10.3390/cells14010056

Figure Lengend Snippet: List of antibodies.

Article Snippet: Mouse anti-MAP-2 (Clone AP20, 1:500) , Millipore (Darmstadt, Germany) , MAB3418.

Techniques: Transduction